Comparison of antibacterial effect of three endodontic sealers; AD seal, AH plus, Endofill on Entrococcus Faecalis after 3, 5 , 7 days (An In vitro study)

Abstract

Antimicrobial properties of endodontic sealers would influence the success rate of root canal therapy. Therefore, this study aimed to compare the antimicrobial effect of Adseal, AH plus, and Endofill sealers on Enterococcus faecalis by direct contact test (DCT). Methods and Materials: Sixty-eight plates were cultured with E. faecalis suspension(1/5*108 CFU/ml) on Muller Hinton agar medium supplemented with 5% sheep blood . Four pits were prepared on medium surface by metal punch and the freshly mixed sealers were applied to three of them. The fourth pit was maintained empty as positive control. After incubation at 37C and 5% CO2 , the diameter of the bacterial growth-free area around sealers was measured at 3, 5, and 7-day intervals. Results: Kruskal-Wallis analysis revealed that there was a significant difference in growth-inhibition area grade among three experimental sealers grades at all study intervals (p<0.01). At all intervals, Endofill beard more growth-inhibition diameter in comparison to two other sealers (p<0.01). Conclusions: Endofill sealer have more antibacterial effect rather than Adseal and AHplus on Enterococcus faecalis. The antibacterial effect of sealers ceases as time elapses.