Evaluation of the effects of mesenchymal stem cell-derived vesicles on differentiation and function of peripheral blood TH17 cells from patients with COVID-19 disease

Abstract

Inhibition of cytokine storm along with the inhibition of proliferation of the virus could be an effective therapeutic approach in the treatment of COVID-19 patients. In this study, we evaluate the effects of MSC exosomes on PBMCs of COVID-19 patients. Materials and Method In this experimental study, 5 ccs of blood containing anticoagulant was taken from 20 covid-19 patients in order to isolate PBMC. After sampling and isolating mononuclear cells (PBMC) from them, the level of gene expression and levels of IL-17, IL-22, and IL-23 was measured. Differentiation of lymphocytes into TH17 was also measured. Finally, GraphPad Prism software was used for data analysis, and p<0.05 is considered as a significant level. Results: Based on our results, the expression level of the cytokines gene in covid-19 patients was lower than in the control group. The differentiation rate of lymphocytes to TH17 was decreased dramatically after receiving MSC exosomes.