Effect of metabotropic glutamate receptors II /III agonists on spike-wave discharge in primary somatosensory perioral cortex of male WAG/Rij rats

Abstract

The aim of this study was investigation of metabotropic glutamate receptors II/ III on spike-and-wave discharges in perioral somatosensory cortex of male WAG/Rij rats. Eighteen male WAG/Rij rats was used in this experiment. Rats anesthetized with a combination of ketamine and xilusinip and 2 ?l of drugs injected with a 10 ?l Hamilton syringe bilaterally. At the end of the experiment, all animals were killed by high dose of ketamine. The brain was removed rapidly and fixed in 10% formalin. Cresyl violet stained coronal sections were viewed with a light microscope to verify cannula placement. In all groups mean numbers and mean duration of Spike-and-wave discharges was decreased, but peak frequency was only in L-AP4 group was reduced. These agonists are able to suppress epileptic discharges in WAG/Rij rats and could be useful for treatment of Absence epilepsy in the future.