In vitro evaluation of the antimicrobial activity of liposome-encapsulated piperacillin/tazobactam against Pseudomonas aeruginosa strains

Abstract

Background and Aim: Pseudomonas aeruginosa is a gram-negative, encapsulated and opportunistic bacterium. This bacterium has low growth requirements and high antibiotic resistance to antibiotics, which is associated with biofilm production. The various factors that make pathogens resistant to multiple drugs are a common health concern and challenge the high cost of treatment. The aim of this study was to evaluate the antimicrobial effect of the liposomal form of piperacillin and piperacillin / tazobactam in order to overcome the drug resistance of P. aeruginosa. Materials and Methods: In this study, the effect of free and liposomal forms of piperacillin / tazobactam and piperacillin sodium against clinical isolates of P. aeruginosa was investigated. Therefore, different forms of antibiotics were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm eradication concentration (MBEC) and motility inhibition tests on bacterial isolates. Results: Our experiment showed improvement in the liposomal form of Piperacillin sodium. Because MICs and MBCs were lower in liposomal form than in free form. Also, the liposomal form of piperacillin sodium performed better at lower doses than the free form against biofilm, and biofilm eradication in P. aeruginosa occurred at lower doses. In addition, the liposomal form of the drug is able to inhibit bacterial motility. Conclusion: The results showed that the liposomal form of the antibiotic piperacillin sodium works better than the free form, but the results of the liposomal form of piperacillin / tazobactam did not have better results than the free form.