Molecular Characteristics of Staphylococcus aureus Isolates from Buffaloes Milk

Abstract

The aim of the present study was to analyse the aroA and X region of spa genes on Staphylococcus aureus isolates collected from milks of native buffaloes in north-west of Iran. For this purpose, seventy five S. aureus isolates were examined by PCR-RFLP. Amplification of AroA gene revealed a single amplicon with a size of approximately 1,153 bp, but restriction profile analysis of this amplicon yielded three different genotypes including genotype A (9.3%), B (88%) and N (2.7%). Amplification of X region of the spa gene showed an amplicon of 1110; 1130; 1160; 1190 bp in 19, 15, 12 and 29 isolates, respectively. HhaI digestion of this amplicons demonstrated that the isolates having the same PCR band size were in the same PCR-RFLP patterns and there was not variation into isolates belonged to the same PCR amplicon.