Promoters of cancer genes for recombinant protein expression in human cancer cell lines

Abstract

Introduction: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges. Methods: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors. Results: After designing, these vectors could be applied to produce complex human recombinant proteins in the human cancer cell lines as production hosts. Conclusion: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields. آ© 2012 by Tabriz University of Medical Sciences.