The Investigation of radiation effect on MDA oxidative stress and IL-1β cytokine in diabetic and healthy lymphocyte cells

Abstract

In this study, difference between healthy and diabetic groups in response to different doses of radiation on the cell viability and secretion of IL-1β, MDA factors were studied.

Materials and Methods: Peripheral blood lymphocyte cells of diabetics (Glucose level A=260, B=287 mg/dl) and healthy individual (Glucose level <100 mg/dl) were exposed to X-rays (2, 4 and 8 Gy). The analysis of cell viability by MTT, MDA by spectrophotometry and IL-1β by ELISA assay were performed for normal/diabetic exposed sample cells.

Results: Data showed that there was a significant correlation between percentage of cell viability in normal groups (100, 85, 76 and 67) with diabetic groups A (100, 73, 64 and 50) and diabetic group B (100, 74, 59 and 48) in response to different dose of radiation (0, 2, 4, and 8 Gy), respectively (P<0.01). Study of data analysis of IL-1β levels was statistically significant in the normal group (10, 12, 20 and 32) with diabetic groups A (18, 21, 28 and 64) and diabetic B groups (16, 24, 34 and 70) in response to different radiation dose (0, 2, 4 and 8 Gy), respectively (P<0.001). Analysis of data on MDA levels showed a significant correlation between normal group (0.8, 0.8, 0.9 and 1) with diabetic groups A (1.5, 1.9, 2.2 and 2.5) and diabetic group B (1.6, 1.8, 2 and 2.4) in radiation dose (0, 2, 4 and 8 Gy), respectivily (P<0.05).