Detemination of aspitan (Fluvalinate) residue in honey and bee,s wax samples of Tabriz market

Abstract

Introduction: Fluvalinate is a pesticide, placed in honey bee hives to prevent acarids and pests infestation. A strip containing 10% fluvalinate, starts to affect in 6 to 8 weeks exposed to honeycombs. Expoture to this toxin can cause irritation, burning, headache, nausea etc. Aim: The aim of the present study is to detect and assay fluvalinate in samples of honey and beeswax, marketed in Tabriz city. Materials and Methods: In this study, 19 samples, including 14 samples of honeydew and 5 samples of beeswax were collected and assayed, using a new DLLME method coupled GC-FID and GC-MS techniques. The fluvalinate in the samples was first extracted by octanol and then concentrated by a liquid-liquid micro extraction method (DLLME). Results: Findings suggest that no toxin i.e. Apasitan and its possible interferers (Fenarimol, Tetradifone, Chorothalonill, Buprofezin, Molinate),were found in any of the honey samples abtained from local marceket. Discussion: in the analysis of the samples, GC-MS method was used to confirm the results of the assay by GC-FID, results of which proved no fluvalinate-infestation in studied honey samples.