Response of human T cells to tetanus neurotoxin HCC Sub-Domain: T cell cytokine production and activation marker induced by HCC(Article)
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Abstract
Tetanus is caused by the tetanus neurotoxin (TeNT), a 150 kDa single polypeptide molecule which is cleaved into active two-chain molecules composed of a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy (H) chains. Fragment C is further subdivided into two subdomains: the proximal H<inf>CN</inf> subdomain and the extreme carboxy subdomain, H<inf>CC</inf>. H<inf>CC</inf> is considered as an immunodominant part of TeNT and is responsible for TeNT binding activity to neurons. In the present study, we investigated the ability of recombinant H<inf>CC</inf>(r H<inf>CC</inf>) to induce T cell activation. Our results showed that recombinant H<inf>CC</inf> has a stimulatory effect on IFN-? secretion by T cells after 48h co-incubation in the presence of anti-TLR-2 Ab. Also, Hcc can induce the expression of CD69 on T cells. Our finding indicated that stimulatory effects of H<inf>CC</inf> on T cells are TLR-2 independent and anti-TLR-2 inhibitory antibody fails to neutralize H<inf>CC</inf> stimulatory effects on T cells. Furthermore, H<inf>CC</inf> is critical for immunogenic activity of TeNT and is able to induce T cells through TLR-2 independent pathway. Copyright é Autumn 2015, Iran J Allergy Asthma Immunol. All rights reserved.
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CD3 antigen, CD69 antigen, gamma interferon, interleukin 4, neurotoxin, T lymphocyte antibody, tetanus neurotoxin hcc subdomain, tetanus toxin, toll like receptor 2, tumor necrosis factor alpha, unclassified drug, antibody, CD69 antigen, gamma interferon, IL4 protein, human, interleukin 4, lectin, leukocyte antigen, neuromuscular blocking agent, peptide fragment, T lymphocyte antigen, tetanus toxin, tetanus toxin fragment C, TLR2 protein, human, TNF protein, human, toll like receptor 2, tumor necrosis factor alpha, Article, B lymphocyte, binding affinity, carboxy terminal sequence, CD4+ T lymphocyte, cellular immunity, controlled study, cytokine production, cytokine release, helper cell, human, human cell, immunogenicity, nerve cell, peripheral blood mononuclear cell, protein expression, T lymphocyte activation, drug effects, immunology, lymphocyte activation, secretion (process), T lymphocyte, Antibodies, Antigens, CD, Antigens, Differentiation, T-Lymphocyte, Humans, Interferon-gamma, Interleukin-4, Lectins, C-Type, Lymphocyte Activation, Neuromuscular Blocking Agents, Peptide Fragments, T-Lymphocytes, Tetanus Toxin, Toll-Like Receptor 2, Tumor Necrosis Factor-alpha