Detection of t(8;14) c-myc/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell lymphoma
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Abstract
Objective/background Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL. Methods In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and ? switch region) and the myc gene (Exons 2 and 3). Results As much as 1.85% of the samples were positive for the ? constant region and Exon 2 of the myc gene. Conclusion LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL. é 2016
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immunoglobulin heavy chain, ethidium, Myc protein, Article, cancer patient, chromosome translocation, constant region, controlled study, DG75 cell line, exon, gene amplification, gene rearrangement, gene structure, genetic analysis, genetic association, histopathology, human, human cell, human tissue, IgH gene, joining region, large cell lymphoma, long distance polymerase chain reaction, lymphoma cell line, oncogene c myc, phenotype, polymerase chain reaction, Raji cell line, touch down polymerase chain reaction, agar gel electrophoresis, chromosome 14, chromosome 8, chromosome breakage, diffuse large B cell lymphoma, gene rearrangement, gene translocation, genetics, metabolism, polymerase chain reaction, procedures, tumor cell line, Cell Line, Tumor, Chromosome Breakage, Chromosomes, Human, Pair 14, Chromosomes, Human, Pair 8, Electrophoresis, Agar Gel, Ethidium, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Humans, Lymphoma, Large B-Cell, Diffuse, Polymerase Chain Reaction, Proto-Oncogene Proteins c-myc, Translocation, Genetic