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Study of the Concentration of Stool Calprotectin in Children with Cystic Fibrosis
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Hejrati, Suzan; Jafari Rouhi, Amir Hosein; Rafeey, Mandana
The aim of this study was to evaluate the FC level in patients with CF and its relationship with various factors such as disease severity. Methods: 52 children with cystic fibrosis were enrolled in the study and data such as age, sex, weight, age at diagnosis, pancreatic insufficiency, respiratory symptoms, and pulmonary infections and respiratory failure were recorded. Also, fecal calprotectin level was measured by ELISA in all patients. Then, the correlation between fecal calprotectin level in patients with each of the above cases was evaluated. Results: The mean age of the patients was 8.85±5.05 years and at the time of diagnosis were 13.49±3.4 months. Mean FC of patients was 60.32±34.53 µg / dl. 22 patients (42.3%) had normal FC and 30 patients (57.7%) had higher FC than normal. 95.5% (21) of patients with normal FC and 100% (30) of patients with abnormal FC had pancreatic insufficiency (P=0.42). The frequency of exacerbation in patients with normal FC was 2.13±0.74 and in patients with abnormal FC was 5.56±8.67 times (P = 0.08). FEV1 in patients with normal FC was 82.38 9 9.53% and in patients with abnormal FC was 78.14±13.67% (P = 0.25).
Preparation and Evaluation of new Root repair material containing Hydroxyapatite nanoparticles on human dental pulp stem cells
(Tabriz University of Medical Sciences, School of Dentistry, 2021) Mahdilouy tazeh kandi, Mahsa; maleki, solmaz; sharifi, simin; shahi, shahriar
Introduction: Over the past few decades, nanoparticles have been attracted enormous attention due to their functional properties. Nano-hydroxyapatite (nHA) is structurally similar to dental hydroxyapatite and has notable effects on the differentiation of mesenchymal stem cells. Portland cement is a main component of MTA and has similar physicochemical properties with calcium silicate-based cements. The aim of this study was to prepare a new root repair material including portland cement, bismuth oxide and nano-hydroxyapatite and analyze the effects of this combination on proliferation and differentiation of human dental pulp stem cells (hDPSCs). Material and methods: bismuth oxide as a radiopaque component and nano-hydroxyapatite particles were added to white Portland cement with 20% and 5% weight ratio, respectively. The viability of hDPSCs was assessed by MTT assay after 1,3 and 7 days. Also, the odontogenic potential of this substance was evaluated by measuring alkaline phosphatase in the culture medium. One way analysis of variance (ANOVA) and Tukey post hoc were used to compare the cell viability and induction of mineralization in the studied groups. P<0/05 was considered statistically significant. Results: The cell viability was significantly increased in new root repair material containing hydroxyapatite nanoparticles after 3 and 7 days (p <0.05). Moreover, Alkaline phosphatase activity increased over a 7-day period in all experimental groups. However, this increase was higher in new root repair material and MTA compared to other groups (p <0.05). Conclusion: Taken together, our experimental results suggest that new root repair material containing nano-hydroxyapatite particles is favorable material and increased alkaline phosphatase activity as well as MTA in hDPSCs; while cell viability was higher in new root repair material in all experiment days.
Development and Psychometric Assessment of Instrument “ Self-efficacy for Patients with Beta Thalassemia Major”
(Tabriz University of Medical Sciences, School of Nursing and Midwifery, 2021) Hasani Narenjbaghi, Soghra; Ghahramanian, Akram; Asghari Jafarabadi, Mohammad; Nursing; Valizadeh, Leila; Zamanzadeh, Vahid
Abstract Introduction: At present, chronic diseases such as thalassemia is one of the important challenges of the health care system. Self-care is the most important factor which could influence patients' quality of life and it could reduce anxiety, depression, and fatigue of patients with thalassemia. Self-efficacy is an important prerequisite for successful self-care and behavior change. Also, it is the most important determinant of self-care behaviors. Given the importance of the self-efficacy construct, the challenge in studying self-efficacy in patients with thalassemia major is the lack of specific tools for measuring it. Therefore, this study was conducted with the aim of designing and psychometric evaluation the self-efficacy tool for patients with thalassemia major. Method: It was a mixed method study with a sequential exploratory design. In the first stage, a qualitative study with conventional content analysis was conducted to explain the self-efficacy and its dimensions in patients with thalassemia major. Data were collected using unstructured interviews. The research sample included 16 patients with thalassemia major who were selected using a purposeful sampling method. In the next stage, items of the tool were developed using the findings of the qualitative stage and reviewing literature. Then, the face validity of the tool was checked and its content validity was examined through determining the content validity ratio and index. Also, the tool's construct validity was checked using the exploratory factor analysis with the participation of 199 patients with thalassemia major, and its convergent validity was considered using the Rosenberg self-esteem tool with the participation of 102 patients. The tool's reliability was assessed by the internal consistency reliability, and the stability was checked by the test-retest method with the cooperation of 23 patients with thalassemia major in an interval of 2 weeks. Results: Analysis of the qualitative data led to the identification of four categories; including ability belief of "self-acceptance", "change", "activites, management", and "disease acceptance". After explaining the concept of self-efficacy of patients with thalassemia major, the tool draft was prepared with 54 items on a six-point Likert scale. After determining its face validity and content validity, a tool with 37 items was prepared for the next step which was determining the tool's construct validity. In exploratory factor analysis, 2 items with a common variance of less than 0.2 with other items; were removed from the tool. Finally, a tool with 35 items and three factors including "self-acceptance," "adaptation belief," and "treatment adherence/disease control belief" was designed. The cumulative variance of the three factors was 49.89%. The convergent construct validity was also determined by comparing the designed tool with Rosenberg self-esteem tool and calculating the Pearson correlation coefficient (r = 0.38). The internal consistency reliability of the whole scale was confirmed with Cronbach's alpha coefficient (α) of 0.93 and the intra-class correlation coefficient (ICC) of 0.94. Conclusion: The results showed that patients with thalassemia major who are self-efficient have belief in accepting their limitations and abilities. The self-efficient patient who has self-acceptance; believes in using the strategies of coping, adapts with the society's view towards the disease and himself/herself, as well as the changes resulting from the disease in his/her appearance. In addition, the self-efficient patient with thalassemia major believes in the ability to adhere the treatment to prevent and control the complications of the disease, so that he/she could actively participate in his/her physical and mental health and could improve his/her quality of life. The "Self-Efficacy Scale for Patients with Thalassemia Major" has appropriate psychometric properties to measure the self-efficacy of these patients.
Study of the effects of graphite and activated carbon on morphological characteristics and the production of phenolic acid and volatile compound of Plantago maritima under in vitro culture conditions
(Tabriz University of Medical Sciences and Health services, School of Pharmacy, 2021) Najafi, Fatemeh; Kosari Nasab, Morteza; Asgharian, Parina; Delazar, Abbas
Introduction: The plants of Plantaginaceae family are valuable plants due to the production of secondary metabolites. Today, the production of secondary metabolites using biotechnology is increasing and the plant cell culture enriched with elicitors is a good alternative for material extraction. Goal: The objectives of this study were to investigate the effects of Graphite (G) and Activated charcoal (AC) on the morphological characteristics and phenolic acids and volatile compounds of Plantago maritima in cell culture. Material & metods: Seedlings Plantago maritima cultured in MS medium with different concentrations of G and AC (0, 2, 4, 8 g/L) either individually. After, growth parameters, amounts of total phenol, total flavonoids, antioxidants and photosynthetic pigments were evaluated. LC-MS and GC-MS methods were used to assessment phenolic acids and volatile compounds, respectively. Results: The results indicated that G at high concentrations (4 and 8 g/L) and AC at low concentrations (2 and 4 g/L) led to a considerable rise the morphological characteristics compared to the control (P <0.05). Treatmentss with high concentrations of AC (4 and 8 g/L) had the most total phenol and antioxidant activity, whereas the highest amount of pigments was observed in the treatment of 2 g/l G. According to the results of GC-MS, non-terpenoid compounds had the highest amount of compounds in the control and all treatments. The results of LC-MS showed that Ferulic acid and Rosmarinic acid were detected in the treatments of 8 g/L AC, G and 4 g/L AC, respectively, mentioned phenolic acids were not observed in the control. Discussion: The optimal concentrations of G and AC in the plant cell culture of Plantago maritima could make positive changes in the production of secondary metabolites and morphological characteristics of the plant.
The investigation of epithelial profile changes with OCT in keratoconus undergoing cross-linking
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Jafari, Ahad; Mirzaei, Mohamad
Keratoconus (KCN) is a noninflammatory and billateraly asymmetric disease occuring during the second and third decades of life. The central or paracentral cornea undergoes progressive thinning and steepening causing irregular astigmatism.Videokeratography, including topography and pachymetry is important for screening of KCN. Generally, there are two approaches to treating keratoconus: slowing the progression of the disease and improving the vision. Mild to moderate keratoconus can be treated with eyeglasses or contact lenses. In progressive cases, corneal collagen cross-linking might be indicated to slow or stop the progression. Cross-linking stiffens the cornea to prevent further shape changes. In this procedure, the corneal epithelial thickness increases. The aim of this study is to investigate of epithelial profile changes in patients undergoing cross-linking. Method: Thirty eyes of 30 patients with keratoconus who were candidates for cross-linking were included. After cross-linking by Dresden method, patients underwent anterior segment OCT again with optovue device to examine the corneal epithelium . Results: The mean age of patients was 21.6 ± 4.1 years, of which 17 were female and 13 were male.The uncorrected visual acuity (UCVA) was 0.67 ± 0.18 before surgery and 0.59 ± 0.21 3 months after surgery(P value = 0.001).The best corrected visual acuity (BCVA) was 0.31 ± 0.05 before surgery and 0.19 ± 0.06 3 months after surgery (P value = 0.01). The superior corneal epithelial thickness was significantly reduced after surgery (55.2±3.8 and 52±3.58, befaore and after surgery, respectively, p value = 0.001). The inferotemporal corneal epithelial thickness was also significantly reduced after surgery (55.05±4.59 and 51.6±3.40, before and after surgery, respectively, p value = 0). The ratio of the superior /inferior corneal thickness was significantly reduced after surgery (1.02±0.069 and 0.966±0.031, before and after surgery, respectively, p value = 0.001).
Therapeutic effectiveness of local Botulinum toxin injection on hand tremor severity and upper limb function in patients with idiopathic Parkinson disease - A Systematic Review
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Fallahmanshadi, Leila; Toopchizadeh Tabrizian, Vahideh; Ghoojazadeh, Morteza; Eslamiyan, Fariba
Parkinson's disease (PD) is the second most common neurodegenerative disorder in the world and the clinical symptom of hand tremor is one of the primary and common motor symptoms of this disease. Tremor may be resistant to oral drug treatments that make its treatment challenging. Therefore, botulinum toxin (BoNT) is a suitable alternative treatment for tremor. The aim of this systematic review study was to investigate and analysis of studies with the efficacy and safety of botulinum toxin (BoNT) local injection on the hand tremor severity and upper extremity function in patients with idiopathic Parkinson's disease. Methods: A systematic comprehensive search was performed from 1990 to December 2020 in electronic databases including Cochrane Central Control Files, MEDLINE (PubMed), Scopus, Web of Science, EMBASE, Google Scholar, Clinicaltrial.gov, ProQuest for English, and SID, Magiran, Irandoc and Iranianmedex texts for Persian literature including all studies that investigated the effect of BoNT local injection on hand tremor control and upper extremity function in Parkinson's patients was performed. Results: In this systematic review study, finally was 10 studies in accordance with all inclusion criteria, which is including 1 randomized double-blinded crossover clinical trial and 9 studies of pilot single-center studies without randomized or open label that were classified as cohort studies. There were 131 patients in all. The mean duration of patient's hand tremor was5.93 ± 2.08 years. The tremor severity in patients ranged from 1 to 3. The injectable dose was between 68 -100 IU of Anabotolinium toxin in each upper extremity. In the studied studies were identified 3 outcome groups (1) The first outcome group includes non-kinematic assessment of hand tremor severity criteria which was evaluated by questionnaires such as Fahn-Tolosa-Marín scale (FTM), UPDRS (Unified Parkinson's Disease Rating Scale) and NIHCGC tremor severity scale. (2) The second outcome group was kinematic evaluation of hand tumor intensity which was measured by installing sensor devices on the affected hand. (3) The third outcome group was evaluated for pain intensity, daily functioning and patient's quality of life, which was evaluated by using with VAS, ADL, manual dynamometer, Ergometer, PDQoL questionnaire, QUEST questionnaire and PGIC (patient global impression) questionnaire. The results of this study showed a decrease in UPDRS_20 and UPDRS_21item, after botulinum toxin injection in the hands of individuals by 1.22 ±1.1 unites and 1.20 ± 0.9 units, respectively. In addition, BoNT local injection is tolerable for many patients without serious complications.
Evaluation of the effect of DMF and Ruxolitinib combinational therapy on Experimental autoimmune encephalomyelitis (EAE) as a muose model of Multiple sclerosis (MS)
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Hosseini, Arezoo; Jadidi Niaragh, Farhad; Ebrahimi Kalan, Abbas; Baradaran, Behzad; Babaloo, Zohreh
Multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE) are chronic neurological disease in young adults that is characterized by inflammation and demyelination of the central nervous system (CNS). The pathogenesis of ms is much more complex process, which is mediated by various immune cells including CD4+ T cells, CD8+ T cells and B cells. Evidence from several studies have implicated CD4+ Th cells play critical role in the pathogenesis of MS. Among various groups of CD4+ Th cells, Th17 cells play an important role in MS. Th17 cells are involved in MS disease severity and activity with secretion of pro-inflammatory cytokines, such as IL-17. Th-17 / Treg balance plays an important role in MS, and in this study we examined the concomitant therapeutic effect of two compounds; Ruxolitinib and DMF that are able to alter this balance in EAE mice. Materials and Methods: C57BL/6 mice (6–8 weeks old) were used in our study. Mice were injected with MOG peptide along with complete freund’s adjuvant and M. tuberculosis. They received the DMF and Ruxolitinib according to the protocol for 14 days. After 14 days of treatment, the mice were sacrificed and the spleen, spinal cord, and brain were removed for testing. The expression proinflammatory and anti-inflammatory cytokines, such as IL-10 and IL-17A, was analyzed by real-time PCR. The frequency of TH17 cells and Tregs was assessed by flow cytometry. Findings: Ruxolitinib and DMF decreased the inflammation and demyelination rate in EAE mouse model. Our results also showed that the percentage of Th-17 cells in CNS decreased significantly in the group treated with combination therapy compared with the control group, while the percentage of Treg cells increased. Inflammatory cytokine levels after combination therapy was significantly lower than in control group, while the IL-10 anti-inflammatory cytokine levels were higher than in the control group.
The Correlation of Efflux Pumps and Porin Proteins with Carbapenem Resistance in Clinical Isolated Acinetobacter baumannii from Sina Hospital-Tabriz
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Vahhabi, Abolfazl; Baradarn, Behzad; Samadi Kafil, Hossein; Hasani, Akbar; Hasani, Alka; Ahangarzadeh Rezaee, Mohammad
Carbapenem resistant Acinetobacter baumannii (CRAB) is increasingly recognized as an important nosocomial pathogen, especially in the intensive care units (ICUs), and has made difficult the treatment of infections. Enzymatic degradation by oxacillinase and metallo β-lactamase is the most common mechanism of resistance to carbapenem, but changes in the expression of efflux pumps and porin proteins can also contribute to this resistance. In this study, different mechanisms of resistance to carbapenem were investigated in A. baumannii clinical isolates. Methods: 112 A. baumannii isolates were collected from clinical specimens of patients admitted to Sina hospital in Tabriz. After agar disk diffusion and MIC determination, PCR method was performed to determine the frequency of oxacillinase and metallo β-lactamase genes and Real-time PCR was performed to determine the expression of adeB, adeJ and carO, omp 33-36, oprD genes. Results: All A. baumannii isolates were resistant to carbapenems and had blaOXA-51 gene. 82.1% of them carried blaOXA-23 gene and 36.6% blaOXA-24 gene. The prevalence of metallo β-lactamase genes was 10.7% including 4.5% blaIMP and 6.2% blaNDM. Overexpression of efflux proteins adeB, adeJ was observed in 66% and 42.8%, respectively, and low expression of porins carO, omp 33-36, oprD was observed in 77.7%, 66.6% and 74% of isolates, respectively.
Evaluation of response rate and prognostic factors in children with refractory status epilepsy treated with intravenous immunoglobulin
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Ebrahimi, Pouya; Shiva, Shadi
Status Epilepsies are one of the most common emergencies in neuroscience that can endanger the patient's life. The main predictors of disease outcome are the patient's age, the cause of the epilepsy and the duration of that. Immune-based therapies, such as intravenous immunoglobulin injections, are used when conventional therapies, such as anticonvulsants, do not work well or there is a high probability of an autoimmune process. Objective: The aim of this study was to evaluate the effect of intravenous immunoglobulin and the findings of predictive indicators in children with refractory status epilepsy. Materials & Methods: In this study, we collected the records of all pediatric patients who referred to Tabriz Children's Hospital with refractory status epilepsy and received intravenous immunoglobulin treatment, during one year. After reviewing the inclusion and exclusion criteria, we entered the data of 30 patients into the study and continued this information collecting until the follow-up of the sixth month after receiving treatment. The obtained information was statistically analyzed and interpreted. Results: 20 boys and 10 girls with a mean age of 2.371 ± 2.371 were studied. The number of seizures varied from once a week to 8 times a day. The number of anti-epileptic drugs prescribed for patients was 4 drugs that did not decrease after treatment and even at follow-up. Regarding the cause of epilepsy, 14 patients were of unknown cause, 7 were genetic causes, 5 were structural causes, 3 were hypoxic encephalopathies and one was due to head trauma complications. There is a significant relationship between the causes of seizures and their types. But due to the large number of unknown causes, it could not be concluded. Imaging results from 14 patients show evidence of a variety of disorders that are significantly associated with the response to immunoglobulin treatment (P = 0.038) and the incidence of disease complications. (P = 0.07) 76.7% of patients developed post-disease complications such as mental retardation and attention-hyperactivity disorder. Antiphospholipid antibodies, if present, indicate a higher risk of complications.
The impact of Metolazone plus Furosemide versus Hydrochlorthiazide Plus Furosemide in Patients with Heart failure and refractory edema: A Randomized clinical trial
(Tabriz University of Medical Sciences, School of Pharmacy, 2021) Ehsani, Delaram; Anamzadeh, Ilghar; Entezari-Maleki, Taher; taban sadeghi, Mohammadreza
Introduction: Cardiovascular diseases are one of the top 5 causes of death in the world today. Despite numerous clinical trials, no cure has been found for acute heart failure. The only way to treat heart failure is to control it with drugs. One of the common medication regimens used to overcome resistant edema is to use a combination of a Henle loop diuretic with a thiazide group. Thiazide drugs with synergistic mechanism enhance the effectiveness of Henle loop diuretics. However, no research has been done on the effect of metolazone on the potentiation of furosemide. Objective: In this study, the main purpose was to evaluate and compare the effectiveness of furosemide-metolazone with furosemide-hydrochlorothiazide treatment regimen in edema resistant patients with congestive heart failure. Methods: This study was performed as a non-blind randomized trial in Shahid Madani Heart Hospital of Tabriz University of Medical Sciences.46 patients with the condition were randomly divided into two groups of 23 (furosemide-hydrochlorothiazide and furosemide-metolazone). Changes in the levels of elements such as sodium, potassium and calcium within 5 days of hospitalization were evaluated. Also, changes in weight, urinary fluid, urea and creatinine in the blood of patients were examined and evaluated during 5 days. Results: The results showed that there was not a significant difference in the measurement parameters between the group of patients treated with furosemide-hydrochlorothiazide regimen and the group of patients treated with furosemide-metolazone regimen. Conclusion: In this study, it can be concluded that metolazone furosemide treatment regimen can be as effective as furosemide hydrochlorothiazide treatment regimen.
Study of the effect of curcumin in patients with primary dysmenorrhea
(Tabriz University of Medical Sciences, Faculty of Pharmacy, 2021) Soleimani, Niloufar; Sarbakhsh, Parvin; Shaseb, Elnaz; Hamdi, Kobra
Introduction: Dysmenorrhea is a common problem experienced by women in their reproductive years. NSAIDs and acetaminophen are the mainstays of drug therapy. Curcumin is the principal curcuminoid of the spice turmeric which have anti-inflammatory and anti-oxidant effects. Inflammation is one of the causes of primary dysmenorrhea. Therefore, in this study, the effect of curcumin on primary dysmenorrhea were evaluated. Objective: evaluation of the effects of curcumin in patients with primary dysmenorrhea Method: This study was a single-blind randomized clinical trial on 80 patients with primary dysmenorrhea. The severity of dysmenorrhea were measured by visual analog scale (VAS) and the need of non-steroidal anti-inflammatory drugs (NSAIDs) was studied at the beginning of the study, one and two months after the intervention. Patients were randomly assigned into two groups of 40 patients (control group and intervention group). Control group received just the first line therapy of dysmenorrhea including NSAIDs and acetaminophen but intervention group received two tablets of 500mg curcuma daily two days before menstruation untill three days after, NSAIDS and acetaminophen for two successive cycles. Data analyzed with SPSS version 23. Results: Pain severity was not significantly different between the control and intervention groups, but in both groups we saw a decrease in pain severity, which was greater in the intervention group. After adjusting for the confounders, the pain severity decreased between the two groups and there was a significant difference(P value<0.05). The number of mefenamic acid and acetaminophen used, decreased one month after the study and there was a significant difference between the two groups(P value=0.033). Intra-group studies show that there was also a significant difference in the severity of pain and the number of ibuprofen use in the intervention group) P value<0.001 and P value=0.002). Conclusion: In our study, curcumin reduced pain severity and the number of mefenamic acid, acetaminophen and ibuprofen used in primary dysmenorrhea.
Use of Virtual Social Networks and its Association with Emotional Intelligence and Academic Achievement among Nursing Students of Tabriz University of Medical Sciences at 2018
(Tabriz University of Medical Sciences, School of Nursing and Midwifery, 2021) Hame Morad, Jamal; Arshadi Bostanabad, Mohammad; Nursing; Namdar Areshtanab, Hossein; Ebrahimi, Hossein
Abstract: Background and aim: Nowadays, virtual social networks has become an imperative tool in the exchange of science, knowledge and technology, and found great popularity between different people, especially students, which can affect their academic achievement. Therefore, this study was conducted to determine the status of using social networks and its relationship with emotional intelligence and academic achievement in nursing students of Tabriz University of Medical Sciences. Material and Methods: The present study is a descriptive-correlational study conducted with the participation of 383 nursing students of Tabriz University of Medical Sciences selected by available method. In this study, demographic characteristics questionnaire and societal social status questionnaire, Gilman model of Siberia Schering's emotional intelligence questionnaire, and a general mean score were used to collect data. Collected data were analyzed using SPSS version 16 and descriptive statistics and inferential statistics. Results: In this study, 205 (53.5%) of the participants were female and the Mean ± SD of age and grade point average (GPA) of the participants were 22.09 ± 3.55 and 16.53 ± 1.60, respectively. The most important reason of students for joining to social networks was communicating with friends, awareness of class news, learning science, entertainment, and finding friend. The mean total score of social media use status was 116.81±17.20 and the overall score of emotional intelligence was 99.27±11.48. Pearson correlation coefficient showed that there is a meaningful statistical relationship between the use of social networks with intelligence and academic achievements variables. Discussion and Conclusion: Considering the results of this study, it can be concluded that the status of using virtual social networks has a negative significant relationship with emotional intelligence and academic achievement variables. In other words, it can be said that by improving students' emotional intelligence, it is possible to avoid the harmful effects of social networks on their academic achievement.
Analysis of BCAS4/hsa-miR-185-5p/SHISA7 competing endogenous RNA axis in late-onset Alzheimer’s disease
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Sabaie, Hani; Najmi Tabrizi, Safa; Rezazadeh, Maryam
Alzheimer’s disease (AD) is a heterogeneous degenerative brain disorder with a rising prevalence worldwide. Emerging evidence points to the possibility that the GABAergic signaling pathway undergoes pathological alterations and contributes to pathological hallmarks of AD. SHISA7 (CKAMP59) is a new member of the SHISA family. It exhibits a direct function in inhibitory synaptic GABAAR regulation. We used bioinformatics and experimental methods in this research to explore competing endogenous RNA (ceRNA) regulation of BCAS4 and SHISA7 in tau pathogenesis and their capacity as peripheral biomarkers linked to an abnormal inflammatory response in AD. Methods: The Gene Expression Omnibus database included two microarray datasets, including information on mRNAs (GSE106241) and miRNAs (GSE157239) from individuals with AD with different degrees of AD-associated neurofibrillary pathology and corresponding controls were downloaded. The limma package in the R software was used to identify differently expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). The ceRNA regulatory axes were built using the co-expression and miRNA-mRNA interactions. Additionally, we used the quantitative polymerase chain reaction technique to examine the expression of the BCAS4/hsa-miR-185-5p/SHISA7 ceRNA axis in the peripheral blood (PB) of fifty AD patients and fifty control subjects. Results: BCAS4 was shown to act as a ceRNA to control the SHISA7 expression throughout AD-associated neurofibrillary pathology in temporal cortex (TC) tissue specimens by sponging hsa-miR-185-5p, based on our bioinformatics study. Furthermore, in PB specimens from individuals suffering from AD and normal controls, we found no substantial differences in BCAS4 expression patterns. SHISA7 expression in AD patients’ PB was found to be reduced, as was the case in the TC. Furthermore, we discovered reduced amounts of hsa-miR-185-5p in AD patients’ PB samples compared to control subjects, unlike in TC tissue, where it had been demonstrated to be overexpressed. Finally, SHISA7 and has-miR-185-5p were shown to be effective in identifying AD patients from healthy controls, according to the receiver operating characteristic (ROC) curve evaluation.
Study of the effect of exosomes derived from prostate cancer cells on molecular markers of endothelial-mesenchymal transition (EndMT) in HUVEC endothelial cell model
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Naderali, Fatemeh; Kazemi, Tohid; Solali, Saeed
Genetic changes play an important role in the development of prostate cancer. In addition to changes in the level of DNA, changes in the expression level of genes are also important in cancer progression. It is understood that the intercommunication between cancer cells and their microenvironment is essential to tumor angiogenesis. Exosomes are extracellular vesicles that are important mediators of intercellular communication and play a role in promoting angiogenesis. However, very little is known about the contribution of breast cancer exosomes to tumorogenesis or whether exosomes can mediate docetaxel cancerous action. Methods: HUVEC cell line was cultured in complete RPMI-1640. Cells were treated for 24 hours with DTX, CoCl2, DTX and CoCl2. Cells were harvested, and RNA extraction and cDNA synthesis were performed from cells and exosomes using standard methods. Expression levels of EndMT genes were analyzed using quantitative real-time PCR (qRT-PCR). Results: We observed a decrease in the expression of endothelial markers gene after treatment with PC3 derived exosomes in normoxic and hypoxic condition. And also the expression of mesanchymal markers were increased by DU145-derived exosomes in treated cells.
The Effect Of Educational Video By Pediatric Dentistry For Pulpotomy Skill Improvement Of Dentistry Students Of Tabriz University of Medical sciences Before First Clinical Treatment
(Tabriz University of Medical Sciences, School of Dentistry, 2021) Gholshan Khalili, Golroo; Kouhsoltan, Maryam; Pediatric dentistry; Ghoreyshizadeh, Arezoo
Introduction: Education in the field of medical sciences is an important process in which practical skills are an important part. The aim of this study was to evaluate the effect of educational video on performing palpitomy skills in improving the palpitomial skills of deciduous molars of dental students of Tabriz University of Medical Sciences in the first clinical treatment. Methods and Materials: In this quasi-experimental study, all students studying dentistry at Tabriz University of Medical Sciences who had a two-practice pediatric unit and the first session of pulpotomy clinical treatment and had previously passed pulpotomy training courses as a prerequisite unit were included in the study. Based on the total grade point average, they were matched and divided into two groups of experimental (n = 44) and control (n = 35). Was analyzed using independent t-test. In the control group after the demonstration and in the experimental group after the demonstration and the training video, palptomy skills were performed. Independent t-test was used to compare the DOPS score between the two groups. SPSSV24 software was used to analyze the data. A probability value less than 0.05 was considered as a significant level. Results: The results showed that there was no significant difference in the score of practical children 1 and the score of admission to the control and experimental groups (P> 0.05), and DOPS scores of pulpotomy skills of the control group after demonstration (94 2 2.48). / 15) is less than the experimental group after demonstration and the use of educational film (17.84 82 1.82) and the difference was statistically significant (P = 0.0001) Conclusion: By controlling the scores of the intervening variables, the use of educational video film has been able to increase the DOPS scores of students' pulpotomy skills.
The Prevalence of Concurrent Acute Coronary Syndrome and their Associated Factors Among Patients Referred to Emergency Department with Early Diagnosis of Stroke
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Khosravi, Helin; Ala, Alireza; Sadeghi-Hokmabadi, Elyar; Shams Vahdati, Samad
A small number of stroke patients may present with concurrent ACS, which may be due to shared risk factors between the two disorders. Objectives. We aimed to evaluate the one-year prevalence of concurrent acute coronary syndrome (ACS) and its associated factors in patients presented with acute cerebral stroke at Imam Reza hospital, Tabriz, Iran. Methods. We included 896 adult patients admitted to the emergency department with a definite stroke diagnosis from 2018 to 2019. Data regarding demographics and previous medical history was collected, and the concurrent occurrence of ACS was assessed. Results. The mean (SD) age of subjects with hemorrhagic stroke was significantly lower than those with ischemic stroke or normal CT finding (p < 0.001). The previous history of oral contraceptive pill consumption and sleep apnea, and Modified Rankin Scale of four and more were more prevalent in those having CT finding of stroke than those with normal CT findings (p= 0.005, p=0.003, and p < 0.001, respectively). The prevalence of concurrent ACS was 1 %. The mean (SD) age and the prevalence of the previous history of ischemic heart disease and atrial fibrillation were significantly higher in subjects with concurrent ACS than those without concurrency (p= 0.035, p=0.023, and p=0.048, respectively). There was no significant association between baseline characteristics and concurrent ACS occurrence in patients with stroke after adjusting for confounders.
Study of the effect of exosomes derived from breast cancer cells on molecular markers of endothelial-mesenchymal transition (EndMT) in HUVEC endothelial cell model
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Niktash, Haleh; Kazemi, Tohid; Solali, Saeed
Genetic changes play an important role in the development of breast cancer. In addition to changes in the level of DNA, changes in the expression level of genes are also important in cancer progression. It is understood that the intercommunication between cancer cells and their microenvironment is essential to tumor angiogenesis. Exosomes are extracellular vesicles that are important mediators of intercellular communication and play a role in promoting angiogenesis. However, very little is known about the contribution of breast cancer exosomes to tumorogenesis or whether exosomes can mediate docetaxel cancerous action. Methods: HUVEC cell line was cultured in complete RPMI-1640. Cells were treated for 24 hours with DTX, CoCl2, DTX and CoCl2. Cells were harvested, and RNA extraction and cDNA synthesis were performed from cells and exosomes using standard methods. Expression levels of EndMT genes were analyzed using quantitative real-time PCR (qRT-PCR). Results: We observed a decrease in the expression of endothelial markers gene after treatment with BT-474 derived exosomes in normoxic and hypoxic condition. And also the expression of mesanchymal markers were increased by MDA-MB-231-derived exosomes in treated cells.
Effect of Ginger (Zingiber officinal) on the prevention of postoperative nausea and vomiting after middle ear surgeries
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Ahmadi, Parvaneh; Eslam Jamad Golzari, Samad; Naderpour, Masoud
Surgeries performed on the middle ear increase the rate of postoperative nausea and vomiting through various mechanisms. The aim of this study was to evaluate the effectiveness of ginger on reducing or possibly eliminating nausea and vomiting after middle ear surgery under general anesthesia. Materials and Methods: This study is a clinical trial that was conducted during 2019 in Imam Reza Hospital. A total of 80 patients were randomly admitted to one of the two study groups. Patients in group G received four ginger capsules (Zintoma, Isfahan Goldaru, Iran), each capsule containing 250 mg of ginger powder. For homogenization with Plaspo capsules, gelatin capsules with mint flavor are impregnated. 1 hour before the operation, patients took the capsules with 30 ml of water. Patients were asked about PONV immediately after surgery and at PACU at intervals of 1,2,4,6,6,12,24 and 48 hours after surgery. The results were compared between the two groups. Results: The results showed that preventive use of Zanhbil to prevent nausea and vomiting after middle ear surgery at all times was significantly better than placebo (p <0.05); This means that ginger extract or Zingiber officinal is useful and effective in preventing nausea and vomiting and can be used as a low-harm drug.
The relationship between color Doppler sonography findings with clinical findings in patients referred for evaluation of lower extremity venous system failure to ultrasound ward of Imam Reza Hospital in 2021
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Irandoust, Amir; Jalili, Javad; Hosseinpour-Feizi, Hamideh; Shakeri-Bavil-Olyaie, Abolhassan
Varicose veins of the lower extremities are the most common vascular disorders in humans that cause serious signs and symptoms in patients and sometimes lead to surgical treatment. The aim of this study was to investigate The relationship between color Doppler sonography findings with clinical findings in patients referred for evaluation of lower extremity venous system failure to ultrasound ward of Imam Reza Hospital in 2021. Materials and Methods: In this descriptive-analytical study, 100 patients who referred for lower extremity venous Doppler ultrasound due to venous insufficiency problems were included in the study. Six patients were excluded due to dissatisfaction to participate in the study. 94 patients were evaluated. Patients were asked questions about demographic information, duration of symptoms, occupation, clinical symptoms, history of internal diseases, history of venous problems, and then after obtaining consent, patients underwent color Doppler ultrasound for bilateral saphenofemoral junction failure, which in Valsalva maneuver and reflux for more than one second in the great saphenous vein are considered positive reflux. Results: The mean age of the patients was 39.76±5.51 years and 53.2% were male. The highest frequency of patients' jobs was related to hairdresser (25.5%) and then employee (23.4%). Two patients also reported a history of venous problems in other organs. The most common clinical symptom observed in patients was varicose veins and then telangiectasia or reticular veins (44.7% and 19.1%, respectively). The mean pain of patients according to VAS scale was 3.80±1.09. In addition, edema +1 was observed in 8 patients. Of the 94 patients studied, 36 (38.3%) had positive reflux during the Valsalva maneuver. In the evaluation of venous Doppler in patients, the most common finding was related to saphenofemoral junction failure (44.45%).
The effect of bone marrow derived c-kit+ stem cells on tracheal responsiveness and levels of T-bet and GATA-3 mRNAs in lung tissue of ovalbumin-sensitized male rats
(Tabriz University of Medical Sciences, Faculty of Medicine, 2021) Heyran, Hossein; Keyhanmanesh, Rana; Ahmadi, Mehdi
The effects of intratracheal administration of c-Kit+ bone marrow cells on tracheal responsiveness, total and differential WBC counts as well as lung pathological changes and the expression levels of Gata3 and T-bet mRNAs were evaluated in this investigation. Methods: Forty male Wistar rats (8-10 weeks old, weighting 200-250 g) were allocated randomly into four experimental groups: healthy rats (control), sensitized rats (OVA), sensitized rats receiving c-Kit− cells (OVA+Kit−) and c-Kit+ cells (OVA+Kit+ group). 10 healthy rats were randomly selected for the isolation of bone marrow-derived Kit+ cells. The assesments were done after 14 days. Resoults: Monitoring of labelled tagged cells confirmed successful engraftment of transplanted cells in pulmonary tissue. The statistically significant increase in tracheal contractility, total white blood cell, eosinophil and neutrophil counts, Gata3 expression and pathological changes were seen in sensitized groups compared to C group (p<0.001) while there was significantly decrease in OVA+ckit+ group compared to OVA and OVA+ckit- groups (p<0.001 to p<0.05). The EC50, number of lymphocytes and T-bet mRNA expression diminished in sensitized rats compared with control animals (p<0.001) which elevated in OVA+ckit+ group compared to OVA and OVA+ckit- groups (p<0.001 to p<0.01).