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dc.contributor.authorJavadi, Aniseh
dc.date.accessioned2021-01-13T10:44:06Z
dc.date.available2021-01-13T10:44:06Z
dc.date.issued2020en_US
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/63317
dc.description.abstractGenetic and epigenetic changes are important factors in the incidence and progression of colorectal cancer (CRC). In this regard, microRNA-199a and genes involved in the process of mitochondrial-related apoptosis can play an important role. In the present study, changes in the expression levels of microRNA-199a-5p and SMAC gene in colon tumor samples and healthy adjacent tissue of patients with CRC as well as in CRC adenocarcinoma SW480 cell line were evaluated and the effect of 5-fluorouracil and oxaloplatin on their expression in the cell line was explored. Methods: A total of 47 tumor and healthy tissue samples were collected from the intestines of patients with CRC during surgery. After extraction of total RNA of the samples, gene expression and microRNA expression were measured by Real-time PCR and compared and analyzed between the two study groups. Results: The relative expression level of microRNA-199a in tumor tissues showed a decrease compared to healthy marginal tissues. Also, the expression of SMAC gene involved in mitochondrial-dependent apoptosis in tumor samples significantly decreased compared to healthy samples (p<0.05). Similar results were observed in CRC cancer cell line. On the other hand, although the administration of 5-fluorouracil and oxaloplatin had no effect on the expression level of microRNA-199a, it was able to significantly increase the expression of the target gene SMAC (p<0.001).en_US
dc.language.isofaen_US
dc.publisherTabriz University of Medical Sciences, Faculty of Medicineen_US
dc.subjectColorectal canceren_US
dc.subjectmicroRNA-199aen_US
dc.subjectApoptosisen_US
dc.subjectSMACen_US
dc.subjectPrognosisen_US
dc.titleEvaluation of the expression levels of microRNA-199a-5p, and SMAC gene in tumoral and healthy colon specimens of patients and in colorectal cancer cell line under treatment of oxaliplatin and 5-FUen_US
dc.typeThesisen_US
dc.contributor.supervisorHashemzadeh, Shahriar
dc.contributor.supervisorBadalzadeh, Reza
dc.identifier.docno609722en_US
dc.identifier.callno9722en_US
dc.description.disciplineMedicineen_US
dc.description.degreeMD Degreeen_US


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