Solution structure of a tethered Lmo2LIM2/Ldb1LID complex
MetadataShow full item record
LIM-only protein 2, Lmo2, is a regulatory protein that is essential for hematopoietic development and inappropriate overexpression of Lmo2 in T-cells contributes to T-cell leukemia. It exerts its functions by mediating protein-protein interactions and nucleating multicomponent transcriptional complexes. Lmo2 interacts with LIM domain binding protein 1 (Ldb1) through the tandem LIM domains of Lmo2 and the LIM interaction domain (LID) of Ldb1. Here, we present the solution structure of the LIM2 domain of Lmo2 bound to Ldb1 LID. The ordered regions of Ldb1 in this complex correspond well with binding hotspots previously defined by mutagenic studies. Comparisons of this Lmo2LIM2-Ldb1LID structure with previously determined structures of the Lmo2/Ldb1LID complexes lead to the conclusion that modular binding of tandem LIM domains in Lmo2 to tandem linear motifs in Ldb1 is accompanied by several disorder-to-order transitions and/or conformational changes in both proteins. © 2012 The Protein Society.
Showing items related by title, author, creator and subject.
Leuconostoc mesenteroides-derived anticancer pharmaceuticals hinder inflammation and cell survival in colon cancer cells by modulating NF-?B/AKT/PTEN/MAPK pathways Zununi Vahed, S; Barzegari, A; Rahbar Saadat, Y; Goreyshi, A; Omidi, Y (2017)Promising results from different studies on the effect of probiotics in cancer prevention and therapy have so far been reported. However, the molecular mechanism of the interaction of probiotics with cancer cells is yet ...
DNA damage response and repair in colorectal cancer: Defects, regulation and therapeutic implications Mirza-Aghazadeh-Attari, M; Darband, SG; Kaviani, M; Mihanfar, A; Aghazadeh Attari, J; Yousefi, B; Majidinia, M (2018)DNA damage response, a key factor involved in maintaining genome integrity and stability, consists of several kinase-dependent signaling pathways, which sense and transduce DNA damage signal. The severity of damage appears ...
Akhoondi, S; Sun, D; Von Der Lehr, N; Apostolidou, S; Klotz, K; Maljukova, A; Cepeda, D; Fiegl, H; Dofou, D; Marth, C; Mueller-Holzner, E; Corcoran, M; Dagnell, M; Nejad, SZ; Nayer, BN; Zali, MR; Hansson, J; Egyhazi, S; Petersson, F; Sangfelt, P; Nordgren, H; Grander, D; Reed, SI; Widschwendter, M; Sangfelt, O; Spruck, C (2007)The ubiquitin-proteasome system is a major regulatory pathway of protein degradation and plays an important role in cellular division. Fbxw7 (or hCdc4), a member of the F-box family of proteins, which are substrate recognition ...