Dietary PUFA Increase Apoptosis in Stomach of Patients with Dyspeptic Symptoms and Infected with H. pylori
MetadataShow full item record
Drug-resistant strains of Helicobacter pylori and poor treatment response are the main reasons for the failure in eradicating it in patients. Polyunsaturated fatty acids (PUFA) have an inhibitory effect on bacterial growth. The aim of this study was to investigate the effect of PUFA in combination with standard triple therapy on apoptosis in H. pylori infected subjects with dyspeptic symptoms. This study was a double-blind clinical trial in which 34 H. pylori infected subjects with dyspeptic symptoms were randomly divided into two groups of 17 patients. The control group received standard triple therapy (amoxicillin, clarithromycin and omeprazole) and the experimental group received the standard therapy and PUFA for two weeks. Gene expression levels of caspase-3, BCL-2 and Bad proteins were studied with real-time PCR, while protein levels were quantified in frozen sections and using immunohistochemistry. Compared with the control group, a significant increase (pآ <آ 0.01) was observed in the expression of caspase-3 and Bad genes and a significant reduction (pآ <آ 0.05) in the expression of Bcl-2 gene. The protein level of active caspase-3 and Bad protein was significantly increased and the level of Bcl-2 protein was significantly decreased (pآ <آ 0.05). The results of this study show that oral administration of PUFA in combination with the standard triple therapy increased apoptosis in H. pylori-infected patients with dyspeptic symptoms. This increase in apoptosis may partly reduce drug resistance in these patients. Our results suggest inclusion of a dietary PUFA containing fatty acid supplement may improve treatment of patients that are refractory to the standard triple therapy. آ© 2017, AOCS.
Showing items related by title, author, creator and subject.
An inverse relation between CagA+ strains of Helicobacter pylori infection and risk of erosive GERD Somi, MH; Fattahi, E; Fouladi, RF; Karimi, M; Bonyadi, R; Baballou, Z (2008)Objective: The aim of this study is investigating the association of Helicobacter pylori (H. pylori) infection and its cytotoxic-associated gene A (cagA) strain with reflux esophagitis. Methods: In a case-control setting ...
Association between Helicobacter pylori, cagA, and vacA Status and Clinical Presentation in Iranian Children Rafeey, M; Ghotaslou, R; Milani, M; Farokhi, N; Ghojazadeh, M (2013)Objective: Seroprevalence of H. pylori infection in Iran exceeds 65% of pediatric population. In this study, we intended to find association between the virulence genes (cagA and vacA) and clinical presentations. Methods: ...
Diversity of helicobacter pylori caga and vaca genes and its relationship with clinical outcomes in Azerbaijan, iran Ghotaslou, R; Milani, M; Akhi, MT; Nahaei, MR; Hasani, A; Hejazi, MS; Meshkini, M (2013)Purpose: The purpose of this research was to analyze cagA and vacA genotypes status in H. pylori isolates and relationship with clinical outcomes. Methods: Gastric biopsy specimens were cultured for H. pylori isolation and ...