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dc.contributor.authorShiri-Shahsavar, MR
dc.contributor.authorMirshafiee, A
dc.contributor.authorParastouei, K
dc.contributor.authorEbrahimi-Kalan, A
dc.contributor.authorYekaninejad, S
dc.contributor.authorSoleymani, F
dc.contributor.authorChahardoli, R
dc.contributor.authorMazaheri Nezhad Fard, R
dc.contributor.authorSaboor-Yaraghi, AA
dc.date.accessioned2018-08-26T08:32:32Z
dc.date.available2018-08-26T08:32:32Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/52303
dc.description.abstractVitamins are immunologically interesting due to their significant immunomodulatory activities. Experimental autoimmune encephalomyelitis (EAE) is one of the most commonly used experimental models for studying autoimmune disorder in multiple sclerosis (MS). The aim of this study was to evaluate the protective and ameliorative effects of novel combination of all-trans retinoic acid (ATRA), 1,25-dihydroxyvitamin D3 (D3), and docosahexaenoic acid (DHA) on EAE-specific determinants and target gene expressions. Mice were randomly categorized into three groups before EAE induction [non-treated EAE (Group E), treated EAE (Group T), and healthy mice (Group H)]. Encephalomyelitis was induced in female C57BL/6 mice by subcutaneous immunization using commercial kits. Preceding day of EAE induction, combination of ATRA, D3, and DHA was administered with a single IP injection every 48أ¯?آ½h and continued until day 26. Findings of present study showed that administration of vitamins A, D, and DHA significantly decreased average clinical scores, cumulative EAE score, and EAE incidence in Group T, compared to Group E (p values <0.001). Interferon ? secretion in serum and T-bet mRNA expression in splenocytes were significantly reduced (pأ¯?آ½=أ¯?آ½0.004, pأ¯?آ½=أ¯?آ½0.029, respectively) while PPAR? mRNA expression was significantly increased in Group T compared to Group E (pأ¯?آ½=أ¯?آ½0.021). These findings highlighted that ATRA, D3, and DHA combination modulated PPAR? and T-bet gene expression and resulted in decrease in Th1 response and lymphocyte invasion into the central nervous system (CNS) and resultant inflammation. In conclusion, the results of this study suggested the potential use of this intervention in treatment and/or prevention of EAE/MS and probably other Th1 cell-mediated autoimmune diseases. أ¯?آ½ 2016, Springer Science+Business Media New York.
dc.language.isoEnglish
dc.relation.ispartofJournal of Molecular Neuroscience
dc.subjectcalcitriol
dc.subjectdocosahexaenoic acid
dc.subjectgamma interferon
dc.subjectmessenger RNA
dc.subjectperoxisome proliferator activated receptor gamma
dc.subjectretinoic acid
dc.subjecttranscription factor T bet
dc.subjectcalcitriol
dc.subjectdocosahexaenoic acid
dc.subjectdrug combination
dc.subjectgamma interferon
dc.subjectperoxisome proliferator activated receptor gamma
dc.subjectretinoic acid
dc.subjectT box transcription factor
dc.subjectT-box transcription factor TBX21
dc.subjectvitamin
dc.subjectanimal experiment
dc.subjectArticle
dc.subjectcell infiltration
dc.subjectcell invasion
dc.subjectcentral nervous system
dc.subjectcombination drug therapy
dc.subjectcontrolled study
dc.subjectexperimental autoimmune encephalomyelitis
dc.subjectfemale
dc.subjectgene expression
dc.subjectincidence
dc.subjectinflammation
dc.subjectlymphocyte
dc.subjectmouse
dc.subjectneuroprotection
dc.subjectnonhuman
dc.subjectreal time polymerase chain reaction
dc.subjectspleen cell
dc.subjectTh1 cell
dc.subjecttreatment outcome
dc.subjectanimal
dc.subjectblood
dc.subjectC57BL mouse
dc.subjectdrug combination
dc.subjectdrug effects
dc.subjectEncephalomyelitis, Autoimmune, Experimental
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectAnimals
dc.subjectCalcitriol
dc.subjectDocosahexaenoic Acids
dc.subjectDrug Combinations
dc.subjectEncephalomyelitis, Autoimmune, Experimental
dc.subjectFemale
dc.subjectInterferon-gamma
dc.subjectLymphocytes
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectPPAR gamma
dc.subjectT-Box Domain Proteins
dc.subjectTretinoin
dc.subjectVitamins
dc.titleA Novel Combination of Docosahexaenoic Acid, All-Trans Retinoic Acid, and 1, 25-Dihydroxyvitamin D3 Reduces T-Bet Gene Expression, Serum Interferon Gamma, and Clinical Scores but Promotes PPAR? Gene Expression in Experimental Autoimmune Encephalomyelitis
dc.typeArticle
dc.citation.volume60
dc.citation.issue4
dc.citation.spage498
dc.citation.epage508
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1007/s12031-016-0834-4


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