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dc.contributor.authorZare-Abdollahi, D
dc.contributor.authorSafari, S
dc.contributor.authorMovafagh, A
dc.contributor.authorRiazi-Isfahani, S
dc.contributor.authorGhadyani, M
dc.contributor.authorHashemi-Gorji, F
dc.contributor.authorNasrollahi, MF
dc.contributor.authorOmrani, MD
dc.date.accessioned2018-08-26T08:32:26Z
dc.date.available2018-08-26T08:32:26Z
dc.date.issued2015
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/52291
dc.description.abstractObjectives: Despite numerous studies in order to determine the allele frequency and clinical impact of DNA methyltransferase 3 A (DNMT3A) gene mutations in acute myeloid leukemia (AML), reports about the expression analysis of this gene are rare and between the available, differences are evident. Methods: In this study, we decided to investigate DNMT3A possible expression changes with regard to their mutation and cytogenetic status in a series of 96 AML patients. Results: Mutations were founded in 17 of the 96 patients (17.7%) and associated with higher age and white blood cell count (P < 0.001). Our mutants have had shorter overall survival (OS) (P < 0.001) and relapse-free survival (RFS) (P = 0.011) than those without. Multivariate analysis showed that DNMT3A mutation is an independent prognostic indicator for OS and RFS (P < 0.001). In relation to expression results, we had over and under expression for our favorable and unfavorable cytogenetic subgroups, respectively (P = 0.005 and P < 0.001, respectively). In intermediate subgroup, total DNMT3A expression did not alter (P = 0.575). Interestingly, we noticed similar expression results for DNMT3A transcript 2, to that of the total. Discussion and conclusion: In relation to DNMT3A expression, from the perspective of diagnostic application and its biological significance, it is difficult to accept its primacy over cytogenetic value in favorable and unfavorable subgroups and if so, we did not address this issue in our study due to sample size limitation. In intermediate subgroup, particularly in normal karyotype-AML, given the lack of convincing results, it seems unlikely that DNMT3A expression analysis could attract attention in diagnostic workup and risk prediction of AML. é W.S. Maney & Son Ltd 2015.
dc.language.isoEnglish
dc.relation.ispartofHematology
dc.subjectDNA methyltransferase 3A
dc.subjecthemoglobin
dc.subjectDNA (cytosine 5) methyltransferase
dc.subjectDNA methyltransferase 3A
dc.subjectacute myeloblastic leukemia
dc.subjectadolescent
dc.subjectadult
dc.subjectagar gel electrophoresis
dc.subjectArticle
dc.subjectcancer prognosis
dc.subjectclinical article
dc.subjectcytogenetics
dc.subjectfemale
dc.subjectframeshift mutation
dc.subjectgene expression
dc.subjectgenetic risk
dc.subjectgenetic variability
dc.subjecthuman
dc.subjectleukemia remission
dc.subjectleukocyte count
dc.subjectmale
dc.subjectmiddle aged
dc.subjectmissense mutation
dc.subjectmutational analysis
dc.subjectnonsense mutation
dc.subjectoverall survival
dc.subjectpriority journal
dc.subjectrecurrence free survival
dc.subjectreverse transcription polymerase chain reaction
dc.subjectthrombocyte count
dc.subjecttranscription regulation
dc.subjectyoung adult
dc.subjectage
dc.subjectbiosynthesis
dc.subjectdisease free survival
dc.subjectenzymology
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmortality
dc.subjectmutation
dc.subjectsurvival rate
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAge Factors
dc.subjectDisease-Free Survival
dc.subjectDNA (Cytosine-5-)-Methyltransferase
dc.subjectFemale
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGene Expression Regulation, Leukemic
dc.subjectHumans
dc.subjectLeukemia, Myeloid, Acute
dc.subjectLeukocyte Count
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMutation
dc.subjectSurvival Rate
dc.titleA mutational and expressional analysis of DNMT3A in acute myeloid leukemia cytogenetic subgroups
dc.typeArticle
dc.citation.volume20
dc.citation.issue7
dc.citation.spage397
dc.citation.epage404
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1179/1607845415Y.0000000001


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